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Full length Clone DNA of Human enhancer of zeste homolog 2 (Drosophila) with C terminal Flag tag.
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Full length Clone DNA of Human enhancer of zeste homolog 2 (Drosophila) with C terminal Myc tag.
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Image Search Results
Journal: Aging (Albany NY)
Article Title: Potential value of expression of receptor accessory protein 4 for evaluating the prognosis of lower-grade glioma patients
doi: 10.18632/aging.205695
Figure Lengend Snippet: Methylation level of receptor accessory protein 4 ( REEP4 ) in lower-grade glioma (LGG) based on the TCGA database. ( A ) Methylation sites of REEP4 in LGG. ( B ) The expression level of REEP4 is negatively correlated with the total methylation level. ( C – H ) The expression level of REEP4 is negatively correlated with methylation sites cg13265914, cg16311504, cg07664173, cg20582089, cg19738333 and cg02498268. ( I ) The expression level of REEP4 is positively correlated with the methylation site cg02399048. ( J ) Effect of methylation site cg16311504 on the overall survival of patients with LGG. ( K ) Real-time quantitative polymerase chain reaction showing that the expression level of REEP4 was significantly inhibited by SAM. **** P < 0·0001.
Article Snippet: For the
Techniques: Methylation, Expressing, Real-time Polymerase Chain Reaction
Journal: Frontiers in Oncology
Article Title: Histone Modifications Drive Aberrant Notch3 Expression/Activity and Growth in T-ALL
doi: 10.3389/fonc.2019.00198
Figure Lengend Snippet: GSKJ4 and A-485 treatments modulate Notch receptors expression and activity. Relative NOTCH1, NOTCH3 , and DELTEX1 gene expression (upper panels) and N1ICD, N3ICD, β-actin, H3K27me3, H3K27ac, and H3 total expression levels (lower panels) in: (A) TALL-1 or (C) MOLT3 cells treated for 48 h with 2 μM GSKJ4 or with DMSO. (B) Relative NOTCH1, NOTCH3 , and DELTEX1 gene expression (upper panel) and HA and β-actin protein levels (lower panel) in TALL-1 cells transfected with HA-tagged EZH2 expression vector (HA-EZH2) or with the empty control vector. Relative NOTCH1, NOTCH3 , and DELTEX1 gene expression (upper panels) and N1ICD, N3ICD, β-actin, H3K27me3, H3K27ac, and H3 total expression levels (lower panels) in: (D) TALL-1 or (E) MOLT3 cells treated for 48 h with 5 μM A-485 or DMSO. Data represent mean values of three biological replicates ± Standard Error of the Mean (S.E.M.); ( n = 3) * P < 0.05, ** P < 0.01, *** P < 0.001. Uncropped western blots related to this figure are displayed in .
Article Snippet: The expression vector PIRVNeoSV containing the human c-Myc cDNA coding sequence (c-Myc) was kindly provided by Dr. Giuseppe Giannini (Sapienza University, Rome, Italy). pCMV3-HA vector containing the
Techniques: Expressing, Activity Assay, Transfection, Plasmid Preparation, Western Blot